Composite

Part:BBa_K2598003:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-09-19)


CcaSR under constitutive promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 669
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2315
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1333
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use this part to efficiently sense grren light signals.


Source

CcaSR is obtained from Synechocystis sp. PCC6803

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)

Tabor, J. J., Levskaya, A. & Voigt, C. A. Multichromatic control of gene expression in Escherichia coli. J Mol Biol 405, 315-324, (2011)

Chen, Y. J. et al. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods 10, 659-664, (2013)